产品编号 | bs-17873R |
英文名称 | MTFMT Rabbit pAb |
中文名称 | MTFMT蛋白抗体 |
别 名 | FMT; FMT_HUMAN; FMT1; Methionyl tRNA formyltransferase, mitochondrial; Methionyl-tRNA formyltransferase; mitochondrial; mitochondrial methionyl tRNA formyltransferase; MtFMT. |
研究领域 | 细胞生物 转录调节因子 线粒体 表观遗传学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
克 隆 号 | |
交叉反应 | Mouse (predicted: Human,Rat,Sheep,Cow,Dog) |
产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 44 kDa |
检测分子量 | |
细胞定位 | 细胞浆 线粒体 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human MTFMT: 31-130/389 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
The protein encoded by this nuclear gene localizes to the mitochondrion, where it catalyzes the formylation of methionyl-tRNA. [provided by RefSeq, Jun 2011] Function: Formylates methionyl-tRNA in mitochondria. A single tRNA(Met) gene gives rise to both an initiator and an elongator species via an unknown mechanism. Subcellular Location: Mitochondrion. Similarity: Belongs to the fmt family. SWISS: Q96DP5 Gene ID: 123263 Database links: Entrez Gene: 123263 Human GenBank: NM_139242.2 Human SwissProt: Q96DP5 Human Unigene: 531615 Human |
产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTFMT) Polyclonal Antibody, Unconjugated (bs-17873R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |