| 产品编号 | bs-8477R |
| 英文名称 | MST1/MST2 |
| 中文名称 | 蛋白激酶MST1/2抗体 |
| 别 名 | DKFZp686A2068; FLJ90748; KRS1; KRS2; Mammalian STE20 like protein kinase 1; Mammalian STE20 like protein kinase 2; MST 1; MST 2; MST1; MST2; Serine/threonine kinase 3 (STE20 homolog yeast); Serine/threonine kinase 3 (Ste20 yeast homolog); Serine/threonine kinase 3; Serine/threonine kinase 4; Serine/threonine protein kinase 3; Serine/threonine protein kinase 4; Serine/threonine protein kinase Krs 1; Serine/threonine protein kinase Krs 2; STE20 like kinase MST1; STE20 like kinase MST2; STK 3; STK 4; STK3; STK4; YSK3. |
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Specific References (1) | bs-8477R has been referenced in 1 publications.
[IF=5.589] Gao R et al. PM2.5-associated nitro-PAH exposure promotes tumor cell metastasis through Hippo-YAPmediated transcriptional regulation. Sci Total Environ. 2019 Aug 15;678:611-617. WB ; Human.
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| 研究领域 | 细胞生物 免疫学 信号转导 细胞凋亡 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Zebrafish,GuineaPig,Cat,Chimpanzee,Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropical) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 56kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MST1/MST2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] Function: The human serine/threonine kinase, mammalian STE20 like kinase (MST), is considerably homologous to the budding yeast kinases, SPS1 and STE20, throughout their kinase domains. When stably expressed in HeLa cells, MST highly sensitizes the cells to death receptor mediated apoptosis by accelerating caspase 3 activation. These findings suggest that MST1 and MST2 play a role in apoptosis both upstream and downstream of caspase activation. MST1 is cleaved and activated by caspases during apoptosis and is capable of inducing apoptotic morphological changes such as chromatin condensation upon overexpression. Mammalian Sterile 20 Like 2 (MST2) is most similar to the previously identified MST1 protein kinase (78% identity, 88% similarity). Northern analysis indicates that MST2 mRNA is expressed at high levels in adult kidney, skeletal and placental tissues and at very low levels in adult heart, lung, liver and brain tissues. An in vitro kinase assay indicates that MST2 can phosphorylate an exogenous substrate, as well as itself, and phospho amino acid analysis indicates that it is a serine/threonine protein kinase. Subcellular Location: Cytoplasm. Nucleus. Note: The caspase cleaved form cycles between the nucleus and cytoplasm. SWISS: Q13043 Gene ID: 6788 Database links: Entrez Gene: 6788 Human Entrez Gene: 6789 Human Omim: 604965 Human Omim: 605030 Human SwissProt: Q13043 Human SwissProt: Q13188 Human |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
