产品编号 | bs-17829R |
英文名称 | Rabbit Anti-MRPS35 antibody |
中文名称 | 线粒体核糖体蛋白S35抗体 |
别 名 | 28S ribosomal protein S28; 28S ribosomal protein S28, mitochondrial; 28S ribosomal protein S35; 28S ribosomal protein S35 mitochondrial precursor; 28S ribosomal protein S35, mitochondrial; DKFZp762P093; HDCMD11P; MDS023; MGC104278; mitochondrial; Mitochondrial ribosomal protein S28; Mitochondrial ribosomal protein S35; MRP S28; MRP S35; MRP-S28; MRP-S35; MRPS 28; MRPS 35; MRPS28; MRPS35; OTTHUMP00000240420; OTTHUMP00000240422; OTTHUMP00000240424; PSEC0213; RT35_HUMAN; S28mt. |
研究领域 | 细胞生物 转录调节因子 线粒体 表观遗传学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | (predicted: Human,Mouse,Rat,Dog,Horse) |
产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 37kDa |
细胞定位 | 细胞浆 线粒体 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human MRPS35: 151-250/323 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has had confusing nomenclature in the literature. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 3p, 5q, and 10q. [provided by RefSeq, Jul 2010] Subunit: Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins (By similarity). Subcellular Location: Mitochondrion. SWISS: P82673 Gene ID: 60488 Database links: Entrez Gene: 60488 Human Omim: 611995 Human SwissProt: P82673 Human Unigene: 311072 Human |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |