产品编号 | bs-17824R |
英文名称 | Rabbit Anti-MRPS27 antibody |
中文名称 | 线粒体核糖体蛋白S27抗体 |
别 名 | 28S ribosomal protein S27; 28S ribosomal protein S27, mitochondrial; KIAA0264; Mitochondrial 28S ribosomal protein S27; mitochondrial; mitochondrial ribosomal protein S27; MRP-S27; MRPS27; RT27_HUMAN; S27mt. |
研究领域 | 细胞生物 转录调节因子 线粒体 表观遗传学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | (predicted: Human,Mouse,Rat,Sheep,Cow,Dog,Horse) |
产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 48kDa |
细胞定位 | 细胞浆 线粒体 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human MRPS27: 201-300/414 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2013] Subcellular Location: Mitochondrion. Similarity: Contains 2 PPR (pentatricopeptide) repeats. SWISS: Q92552 Gene ID: 23107 Database links: Entrez Gene: 23107 Human Entrez Gene: 218506 Mouse Omim: 611989 Human SwissProt: Q92552 Human SwissProt: Q8BK72 Mouse Unigene: 482491 Human Unigene: 730225 Human Unigene: 30208 Mouse |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |