| 产品编号 | bs-17809R |
| 英文名称 | MRPS11 Rabbit pAb |
| 中文名称 | 线粒体核糖体蛋白S11抗体 |
| 别 名 | 28S ribosomal protein S11; 28S ribosomal protein S11 mitochondrial; 28S ribosomal protein S11, mitochondrial precursor; Cervical cancer proto oncogene 2 protein; Cervical cancer proto-oncogene 2 protein; HCC 2; HCC-2; mitochondrial; Mitochondrial ribosomal protein S11; MRP S11; MRP-S11; MRPS11; RPMS11; RT11_HUMAN; S11mt. |
| 研究领域 | 细胞生物 信号转导 转录调节因子 细胞类型标志物 线粒体 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 21 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 线粒体 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MRPS11: 61-150/194 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that contains a high level of sequence similarity with ribosomal protein S11P family members. A pseudogene corresponding to this gene is found on chromosome 20. Sequence analysis identified two transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2008] Subcellular Location: Mitochondrion. Similarity: Belongs to the ribosomal protein S11P family. SWISS: P82912 Gene ID: 64963 Database links: Entrez Gene: 64963 Human Entrez Gene: 67994 Mouse Omim: 611977 Human SwissProt: P82912 Human SwissProt: Q9DCA2 Mouse Unigene: 111286 Human Unigene: 297033 Mouse Unigene: 759 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MRPS11) Polyclonal Antibody, Unconjugated (bs-17809R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
