| 产品编号 | bs-7377R |
| 英文名称 | Rabbit Anti-NCK adaptor protein 1/2 antibody |
| 中文名称 | NCK衔接蛋白1/2抗体 |
| 别 名 | Nck 1/2; NCK adaptor protein 1 + 2 GRB4; melanoma NCK protein; MGC12668; NCK; NCK adaptor protein 1; NCK adaptor protein 2; NCK tyrosine kinase; NCKalpha; NCKbeta; NCK1_HUMAN；NCK12_HUMAN； non catalytic region of tyrosine kinase; noncatalytic region of tyrosine kinase, beta; SH2/SH3 adaptor protein NCK alpha; SH2/SH3 adaptor protein NCK beta |
| 研究领域 | 肿瘤 细胞生物 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 43kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NCK adaptor protein 1/2: 2-100/377 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jun 2010] Function: NCK1 is one of the adaptor proteins which mediate specific protein-protein interactions in signaling processes. Adaptor proteins usually contain several domains like SH2 and SH3 which allow specific interactions with other specific proteins. NCK1 and NCK2 show high sequence identity (68%) and have three SH3 domains and a C-terminal SH2 domain. Both of them bind receptor tyrosine kinases such as PDGFR and other tyrosine phosphorylated proteins via their SH2 domains. Various molecules which interact with SH domains of Nck and regulate signaling process of actin cytoskeleton reorganization have been identified. Ncks are thought to have important functions in the development of mesodermal structures during embryogenesis, linked to a role in cell movement and cytoskeletal reorganization. Ncks also have a function in modulating mRNA translation at the level of initiation by interacting eukayotic initiation factor 2 (eIF2). Under stressed conditions, protein synthesis is reduced by inhibiting the activity of eIF2 through phosphorylation, transiently inhibiting recycling of eIF2 into its active form. Subcellular Location: Cytoplasm. Endoplasmic reticulum. Nucleus. Note=Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments. Post-translational modifications: Phosphorylated on Ser and Tyr residues. Phosphorylated in response to activation of EGFR and FcERI. Phosphorylated by activated PDGFRB. Similarity: Contains 1 SH2 domain. Contains 3 SH3 domains. SWISS: O43639 P16333 Gene ID: 4690；8440 Database links: Entrez Gene: 4690 Human Entrez Gene: 8440 Human Entrez Gene: 17973 Mouse Entrez Gene: 17974 Mouse Omim: 600508 Human Omim: 604930 Human SwissProt: O43639 Human SwissProt: P16333 Human SwissProt: Q8BQ28 Mouse SwissProt: Q99M51 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
