| 产品编号 | bs-17611R |
| 英文名称 | CCDC49 Rabbit pAb |
| 中文名称 | 卷曲螺旋结构域蛋白49抗体 |
| 别 名 | Coiled-coil domain-containing protein 49; CWC25_HUMAN; Pre-mRNA-splicing factor CWC25 homolog. |
| 研究领域 | 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 50 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CCDC49: 351-425/425 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Spliceosomes are large ribonucleoproteins that remove introns from nuclear pre-mRNA in a two-step reaction. CCDC49 is a 425 amino acid protein that is involved in the catalytic steps of splicing in Saccharomyces cerevisiae. CCDC49 associates with a component of the Prp19-associated complex, CEF1, which is involved in spliceosome activation. It is likely that CCDC49 facilitates juxtaposition of the 5’ splice site and branch point during the final step in the first catalytic reaction. There are two isoforms of CCDC49 that are produced as a result of alternative splicing events. Similarity: Belongs to the CWC25 family. SWISS: Q6ZUS6 Gene ID: 91050 Database links: Entrez Gene: 91050 Human Entrez Gene: 100503884 Mouse SwissProt: Q6ZUS6 Human Unigene: 106432 Human Unigene: 332379 Mouse |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with CCDC49 polyclonal antibody, unconjugated (bs-17611R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
