| 产品编号 | bs-8767R |
| 英文名称 | Rabbit Anti-WIPI2/Atg21 antibody |
| 中文名称 | 磷酸肌醇相互作用蛋白2抗体 |
| 别 名 | ATG18B; Atg21; CGI 50; DKFZp434J154; DKFZp686P02188; FLJ12979; FLJ14217; FLJ42984; WD repeat domain phosphoinositide-interacting protein 2; WD repeat domain, phosphoinositide interacting 2; WD40 repeat protein interacting with phosphoinositides 2; WIPI 2; WIPI-2; WIPI2; WIPI2_HUMAN; WIPI49 like protein 2; WIPI49-like protein 2. |
| 研究领域 | 细胞生物 信号转导 细胞凋亡 细胞自噬 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat (predicted: Human,Pig,Cow,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 49kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human WIPI2/Atg21: 181-280/454 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM, Mar 2008] Function: Probable early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to PtdIns3P. May bind PtdIns3P. Subunit: Interacts with TECPR1. Subcellular Location: Preautophagosomal structure membrane. Enriched at preautophagosomal structure membranes in response to ptdIns3P. Tissue Specificity: Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors. Similarity: Belongs to the WD repeat SVP1 family. Contains 3 WD repeats. SWISS: Q9Y4P8 Gene ID: 26100 Database links: Entrez Gene: 26100 Human Entrez Gene: 74781 Mouse Omim: 609225 Human SwissProt: Q9Y4P8 Human SwissProt: Q80W47 Mouse Unigene: 122363 Human Unigene: 29432 Mouse Unigene: 163194 Rat |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with WIPI2/Atg21 polyclonal antibody, unconjugated (bs-8767R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
