| 产品编号 | bs-17566R |
| 英文名称 | Rabbit Anti-OTUD5/DUBA antibody |
| 中文名称 | 去泛素酶A抗体 |
| 别 名 | Deubiquinating enzyme A; Deubiquitinating enzyme A; DKFZp761A052; DUBA; MGC104871; OTTHUMP00000025821; OTU domain containing 5; OTU domain containing protein 5; OTU domain-containing protein 5; OTUD 5; otud5; OTUD5_HUMAN. |
| 研究领域 | 细胞生物 转录调节因子 表观遗传学 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit,Chicken) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 61kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human OTUD5/DUBA : 501-571/571 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the OTU (ovarian tumor) domain-containing cysteine protease superfamily. The OTU domain confers deubiquitinase activity and the encoded protein has been shown to suppress the type I interferon-dependent innate immune response by cleaving the polyubiquitin chain from an essential type I interferon adaptor protein. Cleavage results in disassociation of the adaptor protein from a downstream signaling complex and disruption of the type I interferon signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Oct 2008] Function: Negative regulator of innate immune system. Has deubiquitinating activity that is directed towards 'Lys-63'-linked polyubiquitin chains. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production. Subunit: Interacts with TRAF3. Tissue Specificity: Expressed in various tissues, including the liver and placenta, as well as in peripheral blood leukocytes. Post-translational modifications: Phosphorylation at Ser-177 is required for deubiquitinating activity. Similarity: Belongs to the peptidase C85 family. Contains 1 OTU domain. SWISS: Q96G74 Gene ID: 55593 Database links: Entrez Gene: 55593 Human Entrez Gene: 54644 Mouse Omim: 300713 Human SwissProt: Q96G74 Human SwissProt: Q3U2S4 Mouse Unigene: 496098 Human Unigene: 142827 Mouse Unigene: 97794 Rat |
| 产品图片 |
Tissue/cell: human cervical cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-OTUD5 Polyclonal Antibody, Unconjugated(bs-17566R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
