| 产品编号 | bs-10582R |
| 英文名称 | His Tag |
| 中文名称 | His Tag标签抗体 |
| 别 名 | HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag. |
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Specific References (1) | bs-10582R has been referenced in 1 publications.
[IF=6.208] Diana Morais. et al. Liposomal Delivery of Newly Identified Prophage Lysins in a Pseudomonas aeruginosa Model. INT J MOL SCI. 2022 Jan;23(17):10143 WB ; Escherichia coli.
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| 产品类型 | 标签抗体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Recombinant protein |
| 产品应用 | WB=1:2000-5000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated HHHHHH peptide |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. SWISS: N/A Gene ID: N/A |
| 产品图片 |
Sample:
Lane1: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 2ug Lane2: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 2ug Primary: Anti-His tag (bs-10582R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD 
Sample:
Lane 1: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 2ug Lane 2: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4ug Primary: Anti-His tag (bs-10582R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
