产品编号 | bs-17316R |
英文名称 | SGK2 Rabbit pAb |
中文名称 | 丝氨酸/苏氨酸蛋白激酶SGK2抗体 |
别 名 | dJ138B7.2; EC 2.7.11.1; H SGK2; HSGK2; Serine/threonine protein kinase SGK2; Serine/threonine-protein kinase Sgk2; Serum/glucocorticoid regulated kinase 2; Serum/glucocorticoid-regulated kinase 2; SGK2; SGK2_HUMAN. |
研究领域 | 细胞生物 信号转导 激酶和磷酸酶 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
克 隆 号 | |
交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Sheep,Cow,Horse) |
产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 48 kDa |
检测分子量 | |
细胞定位 | 细胞核 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human SGK2: 301-400/427 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
This gene encodes a serine/threonine protein kinase. Although this gene product is similar to serum- and glucocorticoid-induced protein kinase (SGK), this gene is not induced by serum or glucocorticoids. This gene is induced in response to signals that activate phosphatidylinositol 3-kinase, which is also true for SGK. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010] Function: Involved in the activation of potassium channels. Tissue Specificity: Highly expressed in liver, kidney and pancreas, and at lower levels in brain. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. SWISS: Q9HBY8 Gene ID: 10110 Database links: Entrez Gene: 10110 Human Omim: 607589 Human SwissProt: Q9HBY8 Human Unigene: 300863 Human |
产品图片 |
Sample:
ASPC-1(Human) Cell Lysate at 30 ug
RAW264.7(Mouse) Cell Lysate at 30 ug
Primary: Anti-SGK2 (bs-17316R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48/50 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SGK2) Polyclonal Antibody, Unconjugated (bs-17316R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |