| 产品编号 | bs-17271R |
| 英文名称 | SCARA5 Rabbit pAb |
| 中文名称 | 清道夫受体A亚型5抗体 |
| 别 名 | FLJ23907; MGC45780; NET33; SCAR5_HUMAN; SCARA 5; scara5; Scavenger receptor class A member 5; Scavenger receptor class A, member 5(putative); Scavenger receptor class A, member 5; Scavenger receptor hlg; Tesr; Testis expressed scavenger receptor. |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 细胞膜受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat,Cow,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 54 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SCARA5: 231-330/495 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
SCARA5 is a 495 amino acid cell membrane protein. SCARA5 is expressed at high levels in testis, with highest levels specifically found in specific populations of epithelial cells and Sertoli cells. SCARA5 forms a homotrimer single-pass type II membrane complex present on the surface of the plasma membrane. Cells transfected with SCARA5 were able to bind particular gram-positive and gram-negative bacteria, suggesting a possible role for SCARA5 in immunity to infection. It has also been suggested that SCARA5 may be involved in transferrin delivery pathways. Expressed as four isoforms produced by alternative splicing, SCARA5 contains one collagen-like domain and one SRCR domain. Function: Ferritin receptor that mediates non-transferrin-dependent delivery of iron. Mediates cellular uptake of ferritin-bound iron by stimulating ferritin endocytosis from the cell surface with consequent iron delivery within the cell. Delivery of iron to cells by ferritin is required for the development of specific cell types, suggesting the existence of cell type-specific mechanisms of iron traffic in organogenesis, which alternatively utilize transferrin or non-transferrin iron delivery pathways. Ferritin mediates iron uptake in capsule cells of the developing kidney. Binds preferrentially ferritin light chain (FTL) compared to heavy chain (FTH1). Subunit: Homotrimer. Subcellular Location: Cell membrane. Similarity: Belongs to the SCARA5 family. Contains 1 collagen-like domain. Contains 1 SRCR domain. SWISS: Q6ZMJ2 Gene ID: 286133 Database links: Entrez Gene: 286133 Human Entrez Gene: 71145 Mouse Omim: 611306 Human SwissProt: Q6ZMJ2 Human SwissProt: Q8K299 Mouse Unigene: 591833 Human Unigene: 83840 Mouse |
| 产品图片 |
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-SCARA5 (bs-17271R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
Tissue/cell: Mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SCARA5 Polyclonal Antibody, Unconjugated(bs-17271R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
