| 产品编号 | bs-17144R |
| 英文名称 | TRMT12 Rabbit pAb |
| 中文名称 | TRMT12蛋白抗体 |
| 别 名 | alpha amino alpha carboxypropyl transferase TYW2; FLJ20772; Homolog of yeast tRNA methyltransferase; Radical S-adenosyl methionine and flavodoxin domain-containing protein 2; TRM 12; TRM12; TRMT 12; TRMT12; tRNA methyltransferase 12; tRNA methyltransferase 12 homolog; tRNA wybutosine-synthesizing protein 2 homolog; tRNA-yW-synthesizing protein 2; TYW2; TYW2_HUMAN. |
| 研究领域 | 细胞生物 信号转导 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse,Rat (predicted: Human,Pig,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 50 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TRMT12: 231-330/448 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Transfer RNA (tRNA) modifications help regulate the efficiency of mRNA translation by maintaining the correct reading frames. TRM12 (tRNA methyltransferase 12 homolog (S. cerevisiae)), also known as TYW2 (tRNA-yW-synthesizing protein 2) or TRMT12, is a 448 amino acid protein that belongs to the RNA methyltransferase trmD family and TYW2 subfamily. TRM12 is the human homolog of a yeast gene that is essential for the synthesis of yW (wybutosine), a guanosine that stabilizes codon-anticodon associations near the anticodon of phenylalanine tRNA during ribosomal decoding. The gene encoding TRRM12 maps to human chromosome 8, which consists of nearly 146 million base pairs, encodes over 800 genes and is associated with a variety of diseases and malignancies. Schizophrenia, bipolar disorder, Trisomy 8, Pfeiffer syndrome, congenital hypothyroidism, Waardenburg syndrome and some leukemias and lymphomas are thought to occur as a result of defects in specific genes that map to chromosome 8. Function: Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Similarity: Belongs to the RNA methyltransferase trmD family. TYW2 subfamily. SWISS: Q53H54 Gene ID: 55039 Database links: Entrez Gene: 55039 Human Entrez Gene: 68260 Mouse Omim: 611244 Human SwissProt: Q53H54 Human SwissProt: Q8BG71 Mouse Unigene: 9925 Human Unigene: 278805 Mouse |
| 产品图片 |
Sample: Ovary (Mouse) Lysate at 40 ug
Primary: Anti- TRMT12-1124 (bs-17144R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRMT12) Polyclonal Antibody, Unconjugated (bs-17144R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
