| 产品编号 | bs-17123R |
| 英文名称 | TRIM68 Rabbit pAb |
| 中文名称 | TRIM68蛋白抗体 |
| 别 名 | GC109; RNF137; SS-56; SS56; F730114J12Rik; TRI68_HUMAN; TRIM68; RING finger protein 137; RING-type E3 ubiquitin transferase TRIM68; SSA protein SS-56 (SS-56); Tripartite motif-containing protein 68; 2.3.2.27; TRI68_MOUSE; |
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Specific References (1) | bs-17123R has been referenced in 1 publications.
[IF=4.848] Jie Jiang. et al. TRIM68, PIKFYVE, and DYNLL2: The Possible Novel Autophagy- and Immunity-Associated Gene Biomarkers for Osteosarcoma Prognosis. Front Oncol. 2021; 11: 643104 IHC ; Human.
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| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Rat (predicted: Mouse,Pig,Sheep,Cow,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 56 kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TRIM68: 201-300/485 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a protein which contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. This gene is expressed in many cancer cell lines. Its expression in normal tissues, however, was found to be restricted to prostate. This gene was also found to be differentially expressed in androgen-dependent versus androgen-independent prostate cancer cells. [provided by RefSeq, Oct 2011] Function: Functions as an ubiquitin E3 ligase. Acts as a coactivator of androgen receptor (AR) depending on its ubiquitin ligase activity. Subunit: Interacts with AR/androgen receptor (via ligand-binding domain). Interacts with KAT5/TIP60. Subcellular Location: Cytoplasm > perinuclear region. Nucleus. Colocalized with AR in nucleus. Tissue Specificity: Widely expressed. Expressed at high levels in prostate cancer cell lines. Up-regulation could be restricted to androgen-dependent cells. Post-translational modifications: Auto-ubiquitinated. Similarity: Belongs to the TRIM/RBCC family. Contains 1 B box-type zinc finger. Contains 1 B30.2/SPRY domain. Contains 1 RING-type zinc finger. SWISS: Q6AZZ1 Gene ID: 55128 Database links: Entrez Gene: 55128 Human Entrez Gene: 101700 Mouse Omim: 613184 Human SwissProt: Q6AZZ1 Human SwissProt: Q8K243 Mouse Unigene: 523438 Human Unigene: 738828 Human Unigene: 39043 Mouse |
| 产品图片 |
Sample:
HepG2(Human) Cell Lysate at 40 ug
Primary: Anti-TRIM68 (bs-17123R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRIM68) Polyclonal Antibody, Unconjugated (bs-17123R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
