| 产品编号 | bs-17118R |
| 英文名称 | TRIM62 Rabbit pAb |
| 中文名称 | TRIM62蛋白抗体 |
| 别 名 | DEAR1; 6330414G21Rik; TRI62_HUMAN; TRIM62; Tripartite motif-containing protein 62; 2.3.2.27; TRI62_MOUSE; |
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Specific References (1) | bs-17118R has been referenced in 1 publications.
[IF=5.646] Zheng S et al. TRIM6 promotes colorectal cancer cells proliferation and response to thiostrepton by TIS21/FoxM1. J Exp Clin Cancer Res. 2020 Jan 28;39(1):23. IHC-P, Co-IP&ICF ; Human.
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| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat,Rabbit,Sheep,Cow,Dog) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 54 kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TRIM62: 101-200/475 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The tripartite motif (TRIM) family of proteins are characterized by a conserved TRIM domain that includes a coiled-coil region, a B-box type zinc finger, one RING finger and three zinc-binding domains. TRIM58 (tripartite motif containing 58), also known as BIA2, is a 486 amino acid protein that belongs to the TRIM/RBCC family and contains one B box-type zinc finger, a RING-type zinc finger and a single B30.2/SPRY domain. The gene encoding TRIM58 maps to human chromosome 1, which spans 260 million base pairs, contains over 3,000 genes, comprises nearly 8% of the human genome and houses a large number of disease-associated genes, including those that are involved in familial adenomatous polyposis, Stickler syndrome, Parkinson’s disease, Gaucher disease, schizophrenia and Usher syndrome. Similarity: Belongs to the TRIM/RBCC family. Contains 1 B box-type zinc finger. Contains 1 B30.2/SPRY domain. Contains 1 RING-type zinc finger. SWISS: Q9BVG3 Gene ID: 55223 Database links: Entrez Gene: 55223 Human Entrez Gene: 67525 Mouse SwissProt: Q9BVG3 Human SwissProt: Q80V85 Mouse Unigene: 656006 Human Unigene: 30653 Mouse |
| 产品图片 |
Sample: brain (Mouse) Lysate at 40 ug
Primary: Anti-TRIM62(bs-17118R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRIM62) Polyclonal Antibody, Unconjugated (bs-17118R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
