| 产品编号 | bs-17084R |
| 英文名称 | KBTBD11 Rabbit pAb |
| 中文名称 | 慢性粒细胞白血病相关蛋白抗体 |
| 别 名 | KLHDC7C; 2900016B01Rik; 4930465M17Rik; mKIAA0711; RGD1563576; KBTBB_HUMAN; KBTBD11; Chronic myelogenous leukemia-associated protein; Kelch domain-containing protein 7B; CMLAP; KIAA0711; KBTBB_MOUSE; |
| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat,Cow) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 66 kDa |
| 细胞定位 | 细胞核 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KBTBD11: 121-220/623 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The BTB (Broad-Complex, Tramtrack and Bric a brac) domain, also known as the POZ (Poxvirus and Zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. The Kelch repeat and BTB domain-containing protein 11 (KBTBD11) contains 1 BTB (POZ) domain and 4 Kelch repeats suggesting a role in transcription regulation. The gene encoding KBTBD11 maps to chromosome 8, which contains about 800 genes. Translocation of portions of chromosome 8 with amplifications of the c-Myc gene are found in some leukemias and lymphomas. Similarity: Contains 1 BTB (POZ) domain. Contains 4 Kelch repeats. SWISS: O94819 Gene ID: 9920 Database links: Entrez Gene: 9920 Human Entrez Gene: 74901 Mouse SwissProt: O94819 Human SwissProt: Q8BNW9 Mouse Unigene: 5333 Human Unigene: 46675 Mouse |
| 产品图片 |
Sample:
U937(Human) Cell Lysate at 30 ug
Primary: Anti- KBTBD11 (bs-17084R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 66 kD
Observed band size: 63 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KBTBD11) Polyclonal Antibody, Unconjugated (bs-17084R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KBTBD11) Polyclonal Antibody, Unconjugated (bs-17084R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
