| 产品编号 | bs-16980R |
| 英文名称 | KIAA0701 Rabbit pAb |
| 中文名称 | KIAA0701蛋白抗体 |
| 别 名 | Hypothetical protein LOC23074; UH1BL_HUMAN; UHRF1 binding protein 1 like; UHRF1-binding protein 1-like; UHRF1BP1L; KIAA0701.; UHRF1BP1L/SHIP164; |
| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat,Cow) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 161 kDa |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KIAA0701: 1-100/1464 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
UHRF1BP1L (UHRF1 Binding Protein 1 Like) is a Protein Coding gene. An important paralog of this gene is UHRF1BP1. Database links: Entrez Gene: 101139879 Gorilla Entrez Gene: 23074 Human Entrez Gene: 75089 Mouse SwissProt: A0JNW5 Human SwissProt: A2RSJ4 Mouse Unigene: 9724 Cow Unigene: 620701 Human Unigene: 268922 Mouse Unigene: 446134 Mouse |
| 产品图片 |
Protein: brain(mouse) lysate at 40ug;
Primary: rabbit Anti-KIAA0701 (bs-16980R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size:161 kD
Observed band size:161kD
Sample:
Stomach (Mouse) Lysate at 40 ug
Bladder (Mouse) Lysate at 40 ug
Primary: Anti- KIAA0701 (bs-16980R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 161 kD
Observed band size: 161 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-KIAA0701 Polyclonal Antibody, Unconjugated(bs-16980R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
