| 产品编号 | bs-16882R |
| 英文名称 | Rabbit Anti-LACTB antibody |
| 中文名称 | β内酰胺酶样蛋白抗体 |
| 别 名 | FLJ14902; G24; Lactamase, beta; LACTB; Mitochondrial 39S ribosomal protein L56; mitochondrial ribosomal protein L56; MRPL56; LACTB_HUMAN; Serine beta lactamase like protein LACTB; serine beta-lactamase-like protein LACTB, mitochondria; UNQ843/PRO1781. |
| 研究领域 | 细胞生物 信号转导 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 49kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LACTB: 101-200/547 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a mitochondrially-localized protein that has sequence similarity to prokaryotic beta-lactamases. Many of the residues responsible for beta-lactamase activity are not conserved in this protein, suggesting it may have a different enzymatic function. Increased expression of the related mouse gene was found to be associated with obesity. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Dec 2013] Function: LACTB is a protein from the large 39S subunit of the mitochondrial ribosome (mitoribosome). It has some sequence similarity to prokaryotic beta-lactamases but most of the residues that are responsible for the beta-lactamase activity are not conserved between the two proteins. It is expressed predominantly in skeletal muscle. There are two named isoforms. Subcellular Location: Mitochondrion Similarity: Belongs to the peptidase S12 family. SWISS: P83111 Gene ID: 114294 Database links: Entrez Gene: 114294 Human Entrez Gene: 80907 Mouse Omim: 608440 Human SwissProt: P83111 Human SwissProt: Q9EP89 Mouse Unigene: 410388 Human Unigene: 157882 Mouse Unigene: 73451 Rat |
| 产品图片 |
Sample:
liver (Mouse) Lysate at 40 ug
kidney (Mouse) Lysate at 40 ug
Primary: Anti-LACTB (bs-16882R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Sample:
liver (Mouse) Lysate at 40 ug
Primary: Anti-LACTB (bs-16882R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (LACTB) Polyclonal Antibody, Unconjugated (bs-16882R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
