扫码关注公众号           扫码咨询技术支持           扫码咨询技术服务
  
客服热线:400-901-9800  客服QQ:4009019800  技术答疑  技术支持  质量反馈  人才招聘  关于我们  联系我们
产品中心-北京博奥森生物技术有限公司
首页 > 产品中心 > 一抗 > 产品信息
Rabbit Anti-CRHR1  antibody (bs-10248R)
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购QQ:  400-901-9800
技术支持:techsupport@bioss.com.cn
说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-10248R
英文名称 Rabbit Anti-CRHR1  antibody
中文名称 促肾上腺皮质释放激素受体1抗体
别    名 CRF1; Corticotropin releasing hormone receptor variant 1h;CRF-RI; CRF 1; CRF R; CRFR1; CRH R1h; CRF-R1; CRHR 1; CRHR; CRHR1f; Releasing hormone receptor 1; Seven transmembrane helix receptor; corticotropin releasing hormone receptor 1; Crhr; MGC124237; MGC124240; CRF-R; CRF-R-1; CRF-R1; CRFR-1; CRH-R-1; CRH-R1; CRH-R1h; CRHR; CRHR1L; CRFR1_HUMAN.   
Specific References  (1)     |     bs-10248R has been referenced in 1 publications.
[IF=5.273] Hongyu Ma. et al. Corticotropin-releasing factor potentiates glutamatergic input and excitability of presympathetic neurons in the hypothalamus in spontaneously hypertensive rats. NEUROPHARMACOLOGY. 2023 Jun;230:109506  IF ;  Rat.  
研究领域 肿瘤  细胞生物  免疫学  神经生物学  生长因子和激素  内分泌病  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Mouse,Rat (predicted: Human)
产品应用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 48kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CRHR1: 55-150/444 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants one of which is a non-coding read-through transcript with the neighboring gene MGC57346.

Function:
Receptor for corticotropin releasing factor (CRH). Shows high-affinity CRF binding. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.

Subunit:
Interacts (via N-terminal extracellular domain) with CRF and UCN.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Predominantly expressed in the cerebellum, pituitary, cerebral cortex and olfactory lobe.

Post-translational modifications:
C-terminal Ser or Thr residues may be phosphorylated.

Similarity:
Belongs to the G-protein coupled receptor 2 family.

SWISS:
P34998

Gene ID:
1394

Database links:

Entrez Gene: 1394 Human

Entrez Gene: 12921 Mouse

Entrez Gene: 58959 Rat

Omim: 122561 Human

SwissProt: P34998 Human

SwissProt: P35347 Mouse

SwissProt: P35353 Rat

Unigene: 417628 Human

Unigene: 1892 Mouse

Unigene: 10499 Rat



产品图片
Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Cerebellum (Mouse) Lysate at 40 ug Lane 3: Testis (Mouse) Lysate at 40 ug Lane 4: Cerebrum (Rat) Lysate at 40 ug Lane 5: Cerebellum (Rat) Lysate at 40 ug Primary: Anti-CRHR1 (bs-10248R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50-55 kD Observed band size: 48 kD
Tissue/cell: Rat adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min; Incubation: Anti-CRHR1 Polyclonal Antibody, Unconjugated(bs-10248R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRHR1) Polyclonal Antibody, Unconjugated (bs-10248R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRHR1) Polyclonal Antibody, Unconjugated (bs-10248R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
版权所有 2004-2026 www.bioss.com.cn 北京博奥森生物技术有限公司
通过国际质量管理体系ISO 9001:2015 GB/T 19001-2016    证书编号: 00124Q34771R2M/1100
通过国际医疗器械-质量管理体系ISO 13485:2016 GB/T 42061-2022    证书编号: CQC24QY10047R0M/1100
京ICP备05066980号-1         京公网安备110107000727号