| 产品编号 | bs-16788R |
| 英文名称 | KMT1B |
| 中文名称 | 赖氨酸N甲基转移酶1B抗体 |
| 别 名 | FLJ23414; H3 K9 HMTase 2; H3-K9-HMTase 2; Histone H3 K9 methyltransferase 2; Histone H3-K9 methyltransferase 2; Histone lysine N methyltransferase H3 lysine 9 specific 2; Histone lysine N methyltransferase SUV39H2; Histone-lysine N-methyltransferase SUV39H2; KMT1B; Lysine N methyltransferase 1B; Lysine N-methyltransferase 1B; sSuppressor of variegation 3 9 homolog 2 (Drosophila); Su(var)3 9 Drosophila homolog of 2; Su(var)3 9 homolog 2; Su(var)3-9 homolog 2; Suppressor of variegation 3 9 homolog 2; Suppressor of variegation 3-9 homolog 2; Suv39h2; SUV92_HUMAN. |
| 研究领域 | 细胞生物 信号转导 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Horse,Rabbit) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 47kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KMT1B: 311-410/410 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Distinct modifications of histone tails, such as acetylation, phosphorylation and methylation, regulate nuclear processes by organizing chromatin into higher order structures. Higher order chromatin influences chromosome function and epigenetic gene regulation. SUV39H2 is a 410 amino acid protein that localizes to the centromere and contains one SET domain, one pre-SET domain, one post-SET domain and one chromo domain. Expressed at high levels in adult testis, SUV39H2 functions as a histone methyltransferase that trimethylates the Lys-9 residue of Histone H3, thereby playing an essential role in establishing constitutive heterochromatin at pericentric and telomere regions. SUV39H2 conveys its enzymatic activity via its multiple catalytic domains, which are necessary for both stable binding of SUV39H2 to chromatin and for SUV39H2 methyltransferase activity. Multiple isoforms of SUV39H2 exist due to alternative splicing events. Function: Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher order chromatin organization during spermatogenesis. Subcellular Location: Nucleus. Chromosome > centromere. Associates with centromeric constitutive heterochromatin. Similarity: Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. Contains 1 chromo domain. Contains 1 post-SET domain. Contains 1 pre-SET domain. Contains 1 SET domain. SWISS: Q9H5I1 Gene ID: 79723 Database links: Entrez Gene: 79723 Human Entrez Gene: 64707 Mouse Omim: 606503 Human SwissProt: Q9H5I1 Human SwissProt: Q9EQQ0 Mouse Unigene: 554883 Human Unigene: 128273 Mouse Unigene: 23483 Mouse Unigene: 154750 Rat |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
