| 产品编号 | bs-16739R |
| 英文名称 | TRIM45 Rabbit pAb |
| 中文名称 | TRIM45蛋白抗体 |
| 别 名 | FLJ13181; RING finger protein 99; RNF99; TRI45_HUMAN; TRIM45; Tripartite motif containing 45; Tripartite motif containing protein 45; Tripartite motif-containing protein 45. |
| 研究领域 | 细胞生物 转录调节因子 锌指蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Rat (predicted: Mouse,Pig,Sheep,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 64 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TRIM45: 101-200/580 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the tripartite motif family. The encoded protein may function as a transcriptional repressor of the mitogen-activated protein kinase pathway. Alternatively spliced transcript variants have been described.[provided by RefSeq, Mar 2009] Function: May act as a transcriptional repressor in mitogen-activated protein kinase signaling pathway. Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: Expressed in skeletal muscle, brain, heart and pancreas. Similarity: Belongs to the TRIM/RBCC family. Contains 2 B box-type zinc fingers. Contains 1 filamin repeat. Contains 1 RING-type zinc finger. SWISS: Q9H8W5 Gene ID: 80263 Database links: Entrez Gene: 80263 Human Entrez Gene: 229644 Mouse Omim: 609318 Human SwissProt: Q9H8W5 Human SwissProt: Q6PFY8 Mouse Unigene: 301526 Human Unigene: 189351 Mouse Unigene: 102590 Rat |
| 产品图片 |
Sample: Siha Cell (Human) Lysate at 30 ug
Primary: Anti-TRIM45 (bs-16739R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64kD
Observed band size: 64kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRIM45) Polyclonal Antibody, Unconjugated (bs-16739R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
