| 产品编号 | bs-16706R |
| 英文名称 | IRGC Rabbit pAb |
| 中文名称 | IRGC蛋白抗体 |
| 别 名 | CINEMA; IFGGE; IRGC1; Iigp5; R30953_1; F630044M05Rik; Gm1102; Gm474; RGD1311107; IIGP5_HUMAN; IRGC; Immunity-related GTPase cinema 1; 3.6.5.-; IIGP5_MOUSE; IIGP5_RAT; |
| 研究领域 | 细胞生物 免疫学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Rat (predicted: Mouse,Rabbit,Pig,Cow,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 50 kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IRGC: 301-400/463 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
IRGC (Immunity Related GTPase Cinema) is a Protein Coding gene. Diseases associated with IRGC include Solar Retinopathy and Echolalia. GO annotations related to this gene include GTP binding and hydrolase activity, acting on acid anhydrides. An important paralog of this gene is IRGM. Similarity: Belongs to the interferon-inducible GTPase family. SWISS: Q6NXR0 Gene ID: 56269 Database links: Entrez Gene: 56269 Human Entrez Gene: 210145 Mouse SwissProt: Q6NXR0 Human SwissProt: Q8C262 Mouse Unigene: 515444 Human Unigene: 33811 Mouse Unigene: 155129 Rat |
| 产品图片 |
Sample:
A549 Cell (Human) Lysate at 30 ug
Primary: Anti-IRGC (bs-16706R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IRGC Polyclonal Antibody, Unconjugated(bs-16706R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
