| 产品编号 | bs-16619R |
| 英文名称 | Rabbit Anti-IME1 antibody |
| 中文名称 | 减数分裂诱导蛋白IME1抗体 |
| 别 名 | Meiosis inducing protein; IME1_YEAST; YJR094C; Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). 酿酒酵母; |
| 研究领域 | 细胞生物 转录调节因子 表观遗传学 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat (predicted: Saccharomyces cerevisiae) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 42kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Saccharomyces cerevisiae IME1: 301-360/360 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Meiosis-inducing protein (IME1) is a master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p. IME1 is required for sporulation and for early sporulation-specific genes expression. It is a positive regulator of SME1/IME2 expression. Function: Transcription factor required for sporulation and for early sporulation-specific genes expression. Positive regulator of SME1/IME2 expression. Directly activates expression of SLZ1 during meiosis. Subunit: Interacts with UME6. Subcellular Location: Nuclear SWISS: P21190 Gene ID: N/A Database links: Entrez Gene: 853556 Saccharomyces cerevisiae SwissProt: P21190 Saccharomyces cerevisiae |
| 产品图片 |
Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IME1 Polyclonal Antibody, Unconjugated(bs-16619R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
