| 产品编号 | bs-16578R |
| 英文名称 | Rabbit Anti-TRAP5 antibody |
| 中文名称 | 抗酒石酸酸性磷酸酶5型/5型酸性磷酸酶抗体 |
| 别 名 | Tartrate Resistant Acid Phosphatase; Acid phosphatase 5, tartrate resistant; ACP5; EC 3.1.3.2; PPA5_HUMAN; serum band 5 tartrate-resistant acid phosphatase; SPENCDI; Tartrate-resistant acid ATPase; Tartrate-resistant acid phosphatase type 5; TR-AP; TRACP 5; TRAP; TrATPase; Type 5 acid phosphatase. |
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Specific References (2) | bs-16578R has been referenced in 2 publications.
[IF=7.419] Xiaoyu Cai. et al. Secretory phosphoprotein 1 secreted by fibroblast-like synoviocytes promotes osteoclasts formation via PI3K/AKT signaling in collagen-induced arthritis. BIOMED PHARMACOTHER. 2022 Nov;155:113687 FC ; Mouse.
[IF=6.208] Cong-Jin Liu. et al. Preventing Disused Bone Loss through Inhibition of Advanced Glycation End Products. INT J MOL SCI. 2023 Jan;24(5):4953 IF ; Rat.
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| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 淋巴细胞 t-淋巴细胞 b-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Pig) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=2ug/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 34kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TRAP5: 171-270/325 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq, Aug 2008] Function: Defects in ACP5 are the cause of spondyloenchondrodysplasia with immune dysregulation (SPENCDI) [MIM:607944]. A disease characterized by vertebral and metaphyseal dysplasia, spasticity with cerebral calcifications, and strong predisposition to autoimmune diseases. The skeletal dysplasia is characterized by radiolucent and irregular spondylar and metaphyseal lesions that represent islands of chondroid tissue within bone. Note=ACP5 inactivating mutations result in a functional excess of phosphorylated osteopontin causing deregulation of osteopontin signaling and consequential autoimmune disease. Subcellular Location: Lysosome. Similarity: Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family. SWISS: P13686 Gene ID: 54 Database links: Entrez Gene: 54 Human Omim: 171640 Human SwissProt: P13686 Human Unigene: 1211 Human |
| 产品图片 |
Blank control（black line）:Hela.
Primary Antibody (green line): Rabbit Anti-TRAP5 antibody (bs-16578R)
Dilution:2ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
