产品编号 | bs-16465R |
英文名称 | Rabbit Anti-HECTD4 antibody |
中文名称 | HECTD4蛋白抗体 |
别 名 | C12orf51; HECT domain-containing protein 4; HECTD4; HECD4_HUMAN; KIAA0614; Probable E3 ubiquitin-protein ligase HECTD4. |
研究领域 | 细胞生物 泛素 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | (predicted: Human,Mouse,Rat,Sheep) |
产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 439kDa |
细胞定位 | 细胞膜 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human HECTD4: 21-120/3996 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. Function: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (By similarity). Subcellular Location: Membrane; Single-pass membrane protein Similarity: Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. SWISS: Q9Y4D8 Gene ID: 283450 Database links: Entrez Gene: 283450 Human SwissProt: Q9Y4D8 Human |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |