| 产品编号 | bs-16311R |
| 英文名称 | GRF2 Rabbit pAb |
| 中文名称 | G蛋白偶联受体释放因子2抗体 |
| 别 名 | C3G; C3G protein; CRK SH3 binding GNRP; CRK SH3-binding GNRP; DKFZp781P1719; GRF 2; GRF2; Guanine nucleotide releasing factor 2(specific for crk proto oncogene); Guanine nucleotide releasing factor 2; Guanine nucleotide-releasing factor 2; OTTHUMP00000064558; Protein C3G; Rap guanine nucleotide exchange factor(GEF) 1; Rap guanine nucleotide exchange factor 1; RAPGEF 1; RAPGEF1; RPGF1_HUMAN. |
| 研究领域 | 细胞生物 信号转导 G蛋白偶联受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Sheep,Cow) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 140 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GRF2: 20-120/1237 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008] Function: Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Tissue Specificity: Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver. Similarity: Contains 1 N-terminal Ras-GEF domain. Contains 1 Ras-GEF domain. SWISS: Q13905 Gene ID: 2889 Database links: Entrez Gene: 2889 Human Entrez Gene: 107746 Mouse Omim: 600303 Human SwissProt: Q13905 Human Unigene: 127897 Human Unigene: 298274 Mouse |
| 产品图片 |
Sample: Placenta (Mouse) Lysate at 40 ug
Primary: Anti-GRF2 (bs-16311R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 140 kD
Observed band size: 120 kD
Sample:
Lane 1: Human MCF-7 cell lysates
Lane 2: Human HL-60 cell lysates
Lane 3: Human JAR cell lysates
Lane 4: Human HeLa cell lysates
Primary: Anti-GRF2 (bs-16311R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
