| 产品编号 | bs-16271R |
| 英文名称 | Rabbit Anti-GPCR PGR10/GPCR GPR149 antibody |
| 中文名称 | G蛋白偶联受体149抗体 |
| 别 名 | G protein coupled receptor 149; G protein coupled receptor PGR10; GPR 149; GPR149; GP149_HUMAN; IEDA; PGR 10; PGR10; Probable G protein coupled receptor 149. |
| 研究领域 | 神经生物学 G蛋白偶联受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 91kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPCR PGR10/GPCR GPR149: 1-100/731 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
GPR149 (G protein-coupled receptor 149), also known as PGR10 or IEDA, is a 731 amino acid multi-pass membrane protein that functions as an orphan receptor and belongs to the G protein-coupled receptor family. The gene encoding GPR149 maps to human chromosome 3, which houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci. Function: GPCR PGR10 belongs to the G-protein coupled receptor 1 family and is an orphan receptor. PGR10 expression has been documented in rat brain and testis and in chick peripheral nervous system. Subcellular Location: Cell membrane; Multi-pass membrane protein. SWISS: Q86SP6 Gene ID: 344758 Database links: Entrez Gene: 344758 Human SwissProt: Q86SP6 Human Unigene: 688231 Human |
| 产品图片 |
Sample:
Panc-1 (Human)Lysate at 40 ug
MCF-7(Human)Lysate at 40 ug
Primary: Anti-GPCR PGR10/GPCR GPR149(bs-16271R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 91kD
Observed band size: 100kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
