| 产品编号 | bs-16270R |
| 英文名称 | Rabbit Anti-GPCR ORF4/GPCR GPR137 antibody |
| 中文名称 | G蛋白偶联受体137抗体 |
| 别 名 | C11orf4; G protein coupled receptor 137; G protein coupled receptor ORF4; G137A_HUMAN; GPR137; GPR137A; Integral membrane protein GPR137; TM7SF1L1; Transmembrane 7 superfamily member 1 like 1 protein. |
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Specific References (1) | bs-16270R has been referenced in 1 publications.
[IF=5.722] Lin Li. et al. ALKBH1 contributes to renal cell carcinoma progression by reducing N6-methyladenine of GPR137. EUR J CLIN INVEST. 2023 Mar;:e13986 IHC,WB ; Human.
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| 研究领域 | 信号转导 G蛋白偶联受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 46kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPCR ORF4/GPCR GPR137: 201-300/417 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
GPR-137 is a 417 amino acid multi-pass membrane protein that belongs to the GPR-137 family. Existing as three alternatively spliced isoforms, the gene encoding GPR-137 maps to human chromosome 11q13.1. Subcellular Location: Membrane. Similarity: Belongs to the GPR137 family. SWISS: Q96N19 Gene ID: 56834 Database links: Entrez Gene: 56834 Human SwissProt: Q96N19 Human Unigene: 523763 Human |
| 产品图片 |
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GPCR ORF4/GPCR GPR137 Polyclonal Antibody, Unconjugated(bs-16270R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
