| 产品编号 | bs-16108R |
| 英文名称 | FLJ20097 Rabbit pAb |
| 中文名称 | CCDC132抗体 |
| 别 名 | CCDC132; NEDMSC; VPS54L; 1700034M03Rik; 8430415E05Rik; BLM; mKIAA1861; RGD1304751; VPS50_HUMAN; VPS50; Coiled-coil domain-containing protein 132; EARP/GARPII complex subunit VPS50; KIAA1861; VPS50_MOUSE; Bcl2-like protein blm; |
| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 111 kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FLJ20097: 3-100/964 |
| 亚 型 | |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
CCDC132 (coiled-coil domain containing 132) is a 964 amino acid protein that is located on chromosome 7. Two isoforms of CCDC132 exist due to alternative splicing events Tissue Specificity: Ubiquitous, with higher expression in brain and skeletal muscle. SWISS: Q96JG6 Gene ID: 55610 Database links: Entrez Gene: 55610 Human Entrez Gene: 73288 Mouse SwissProt: Q96JG6 Human SwissProt: Q8CI71 Mouse Unigene: 202424 Human Unigene: 222282 Human Unigene: 45539 Mouse Unigene: 1875 Rat |
| 产品图片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- FLJ20097 (bs-16108R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 111 kD
Observed band size: 111 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FLJ20097 Polyclonal Antibody, Unconjugated(bs-16108R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
