| 产品编号 | bs-16104R |
| 英文名称 | TRMT2B Rabbit pAb |
| 中文名称 | X染色体开放阅读框34抗体 |
| 别 名 | Chromosome X open reading frame 34; CXorf34; dJ341D10.3; FLJ12687; Putative methyltransferase CXorf34; TRM2 homolog; TRM2 tRNA methyltransferase 2 homolog B(S. cerevisiae); TRM2 tRNA methyltransferase 2 homolog B; TRM2_HUMAN; tRNA(uracil 5 )methyltransferase homolog; tRNA(uracil(54)-C(5))-methyltransferase homolog; tRNA(uracil-5-)-methyltransferase homolog. |
| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse,Rat (predicted: Human,Rabbit,Chicken,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 56 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TRMT2B: 101-200/504 |
| 亚 型 | |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a homolog of the TRM2 gene in S. cerevisiae. The yeast gene encodes a tRNA methyltransferase that plays a role in tRNA maturation. The yeast protein also has endo-exonuclease activity and may be involved in DNA double strand break repair. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Nov 2009] Function: Probable S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the formation of 5-methyl-uridine at position 54 (m5U54) in all tRNA. May also have a role in tRNA stabilization or maturation. Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. SWISS: Q96GJ1 Gene ID: 79979 Database links: Entrez Gene: 79979 Human GenBank: NM_024917 Human SwissProt: Q96GJ1 Human Unigene: 496501 Human |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with TRMT2B polyclonal antibody, unconjugated (bs-16104R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
