| 产品编号 | bs-16081R |
| 英文名称 | Rabbit Anti-FGD5 antibody |
| 中文名称 | FGD5蛋白抗体 |
| 别 名 | FYVE, RhoGEF and PH domain containing protein 5; ZFYVE23; FGD5_HUMAN; Zinc finger FYVE domain containing protein 23. |
| 研究领域 | 细胞生物 免疫学 信号转导 G蛋白偶联受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat (predicted: Human,Mouse,Dog) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 160kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FGD5: 1301-1462/1462 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
FGD5 is a 1,462 amino acid cytosolic protein that contains a DH domain, a FYVE-type zinc finger and two PH domains. FGD family members encode guanine nucleotide exchange factors that specifically activate the Rho GTPase Cdc42. All FGD proteins contain equivalent signaling domains and a conserved structural organization, which strongly suggests that these signaling domains form a canonical core structure for members of the FGD family of RhoGEF proteins. These proteins also control essential signals required during embryonic development. Function: FGD5 may activate CDC42, a member of the Ras like family of Rho and Rac proteins, by exchanging bound GDP for free GTP. It may also play a role in regulating the actin cytoskeleton and cell shape. FGD5 contains one DH (DBL-homology) domain one FYVE type zinc finger and two PH domains. There are two isoforms. Subcellular Location: Cytoplasm, cytoskeleton. Cell projection, ruffle membrane. Endoplasmic reticulum. Golgi apparatus. Early endosome. Note=In peripheral membrane ruffles, colocolizes with F-actin. In confluent HUVECs, detected at cell-cell-contact sites where it colocalizes with vascular endothelial cadherin/CDH5. Tissue Specificity: Expressed in endothelial cells (at protein level). Similarity: Contains 1 DH (DBL-homology) domain. Contains 1 FYVE-type zinc finger. Contains 2 PH domains. SWISS: Q6ZNL6 Gene ID: 152273 Database links: Entrez Gene: 152273 Human Entrez Gene: 232237 Mouse Omim: 614788 Human SwissProt: Q6ZNL6 Human SwissProt: Q80UZ0 Mouse Unigene: 412406 Human |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FGD5) Polyclonal Antibody, Unconjugated (bs-16081R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
