| 产品编号 | bs-14657R |
| 英文名称 | EXOC1 Rabbit pAb |
| 中文名称 | EXOC1蛋白抗体 |
| 别 名 | BM-012; exoc1; EXOC1_HUMAN; Exocyst complex component 1; Exocyst complex component Sec3; FLJ10893; SEC3; SEC3 like 1; SEC3L1; SEC3P. |
| 研究领域 | 细胞生物 转运蛋白 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat (predicted: Human,Mouse,Pig,Sheep,Cow,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 102 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EXOC1: 701-800/894 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Exocytosis is crucial in membrane trafficking and it mediates hormone and neurotransmitter secretion out of the cell, as well as the incorporation of membrane proteins and lipids to the plasma membrane. It is crucial for cell-cell communication, cell growth and cell polarity. The exocyst complex is a multi-protein complex that consists of Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70 and Exo84, and is essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Exo70, also known as EXOC7 (exocyst complex component 7), EXOC1 or 2-5-3p, is a 735 amino acid peripheral membrane protein that is a component of the exocyst complex. Localized to the cytoplasm and the cell membrane, Exo70 plays an essential role in the docking of exocystic vesicles to target sites on the plasma membrane and, specifically, may be involved in insulin-induced protein docking within the cell. Four isoforms of Exo70 are expressed due to alternative splicing events. Function: Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. Subunit: The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4, EXOC5, EXOC6, EXOC7 and EXOC8. Similarity: Belongs to the SEC3 family. SWISS: Q9NV7O Gene ID: 55763 Database links: Entrez Gene: 55763 Human Entrez Gene: 69940 Mouse Omim: 607879 Human SwissProt: Q9NV70 Human SwissProt: Q9NV7O Human SwissProt: Q8R3S6 Mouse Unigene: 269665 Human Unigene: 256624 Mouse |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EXOC1) Polyclonal Antibody, Unconjugated (bs-14657R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
