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Rabbit Anti-MMP13  antibody (bs-10581R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-10581R
英文名称 MMP13
中文名称 基质金属蛋白酶13抗体
别    名 CLG 3; CLG3; Collagenase 3; Collagenase3; MMP13; MMP 13; MMP-13; Matrix Metalloproteinase 13; MMP 13; MMP13_HUMAN.  
Specific References  (5)     |     bs-10581R has been referenced in 5 publications.
[IF=5.026] Ting-ting Yu. et al. Deletion at an 1q24 locus reveals a critical role of long noncoding RNA DNM3OS in skeletal development. Cell Biosci. 2021 Dec;11(1):1-15  IF ;  Mouse.  
[IF=4.889] Guoqing Li. et al. Teriparatide ameliorates articular cartilage degradation and aberrant subchondral bone remodeling in DMM mice. J ORTHOP TRANSL. 2023 Jan;38:241  IHC ;  Mouse.  
[IF=3.923] Gong X et al. Synergistically regulated spontaneous calcium signaling is attributed to cartilaginous extracellular matrix metabolism. (2018) J. Cell. Physiol. Oct 28.  WB ;  Pig.  
[IF=3.857] Jin S et al. Sequential Epiphyseal Cartilage Changes of Femoral Heads in C57BL/6 Female Mice Treated with Excessive GlucocorticoidsCartilage.2020 Dec 3;1947603520978574.  WB、IHC ;  Mouse.  
[IF=3.386] Tong, Fei. et al. MiR-466d Targeting MMP13 Promotes the Differentiation of Osteoblasts Exposed to a Static Magnetic Field. BIOTECHNOL BIOPROC E. 2023 Feb;:1-10  WB ;  Rat.  
研究领域 肿瘤  心血管  细胞生物  信号转导  细胞骨架  细胞外基质  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse,Rat (predicted: Rabbit)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 52kDa
细胞定位 细胞外基质 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human MMP13: 251-350/471 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008].

Function:
Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.

Subcellular Location:
Secreted, extracellular space, extracellular matrix (Probable).

Tissue Specificity:
Seems to be specific to breast carcinomas.

DISEASE:
Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age. Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia

Similarity:
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.

SWISS:
P45452

Gene ID:
4322

Database links:

Entrez Gene: 403763 Dog

Entrez Gene: 4322 Human

Entrez Gene: 17386 Mouse

Entrez Gene: 171052 Rat

Omim: 600108 Human

SwissProt: P45452 Human

SwissProt: P33435 Mouse

SwissProt: P23097 Rat

Unigene: 2936 Human

Unigene: 5022 Mouse

Unigene: 10997 Rat



产品图片
Sample:
MG63(Human) Cell Lysate at 30 ug
Primary: Anti-MMP13 (bs-10581R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 65 kD
Sample:
Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug
Lane 2: Jurkat (Human) Cell Lysate at 30 ug
Lane 3: MCF-7 (Human) Cell Lysate at 30 ug
Lane 4: Molt-4 (Human) Cell Lysate at 30 ug
Lane 5: A431 (Human) Cell Lysate at 30 ug
Primary: Anti-MMP13 (bs-10581RR) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MMP-13 Polyclonal Antibody, Unconjugated(bs-10581R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (rat lung tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MMP13) Polyclonal Antibody, Unconjugated (bs-10581R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MMP-13 Polyclonal Antibody, Unconjugated(bs-10581R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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