| 产品编号 | bs-14553R |
| 英文名称 | eIF4H Rabbit pAb |
| 中文名称 | eIF4H蛋白抗体 |
| 别 名 | AU018978; D5Ertd355e; E430026L18Rik; Ef4h; eIF 4H; eIF-4H; EIF4H; eukaryotic translation initiation factor 4H; IF4H_HUMAN; KIAA0038; WBSCR1; Williams Beuren syndrome chromosomal region 1 protein homolog; Williams Beuren syndrome chromosome region 1; Willi |
| 研究领域 | 心血管 细胞生物 发育生物学 神经生物学 转运蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse, Orangutan) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 25 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human eIF4H: 31-130/248 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes one of the translation initiation factors, which functions to stimulate the initiation of protein synthesis at the level of mRNA utilization. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008] Function: Stimulates the RNA helicase activity of EIF4A in the translation initiation complex. Binds weakly mRNA. Subcellular Location: Cytoplasm; perinuclear region. Tissue Specificity: The short isoform is the predominant isoform and is expressed alone in liver and skeletal muscle. Both isoforms are expressed in fibroblast, spleen, testis and bone marrow. Levels are high in lung and pancreas and low in heart, frontal cortex and kidney. DISEASE: Note=EIF4H is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of EIF4H may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. Similarity: Contains 1 RRM (RNA recognition motif) domain. SWISS: Q15056 Gene ID: 7458 Database links: Entrez Gene: 7458 Human Entrez Gene: 22384 Mouse Omim: 603431 Human SwissProt: Q15056 Human SwissProt: Q9WUK2 Mouse SwissProt: Q5RBR8 Orangutan Unigene: 520943 Human Unigene: 27955 Mouse Unigene: 79423 Rat |
| 产品图片 |
Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-eIF4H Polyclonal Antibody, Unconjugated(bs-14553R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
