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Rabbit Anti-phospho-EIF4B (Ser406)  antibody (bs-14551R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-14551R
英文名称 phospho-EIF4B (Ser406)
中文名称 磷酸化真核翻译起始因子4B抗体
别    名 eIF4B (phospho S406); p-eIF4B (phospho S406); p-EIF4B (Ser406); p-EIF4B (S406); EIF 4B; EIF-4B; Eukaryotic initiation factor 4B; IF4B_HUMAN.  
产品类型 磷酸化抗体 
研究领域 转运蛋白  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse,Rat (predicted: Dog,Cow,Horse,Sheep)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 69kDa
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human EIF4B around the phosphorylation site of Ser406: HP(p-S)WR 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 eIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot).

Function:
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

Post-translational modifications:
Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.

Similarity:
Contains 1 RRM (RNA recognition motif) domain.

SWISS:
P23588

Gene ID:
1975

Database links:

Entrez Gene: 1975 Human

Entrez Gene: 75705 Mouse

Entrez Gene: 300253 Rat

Omim: 603928 Human

SwissProt: P23588 Human

SwissProt: Q8BGD9 Mouse

Unigene: 648394 Human

Unigene: 702041 Human

Unigene: 290022 Mouse

Unigene: 391795 Mouse

Unigene: 95954 Rat



产品图片
Sample: Testis (Mouse) Lysate at 40 ug
Primary: Anti-phospho-EIF4B (Ser406)(bs-14551R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69kD
Observed band size: 69kD
Sample: Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-phospho-EIF4B (Ser406)(bs-14551R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69kD
Observed band size: 69kD
Sample: Hela Cell (Human) Lysate at 40 ug
Primary: Anti-phospho-EIF4B (Ser406)(bs-14551R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69kD
Observed band size: 69kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-EIF4B (Ser406)) Polyclonal Antibody, Unconjugated (bs-14551R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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