| 产品编号 | bs-14410R |
| 英文名称 | DOPEY2 Rabbit pAb |
| 中文名称 | 迟钝蛋白家族2抗体 |
| 别 名 | 21orf5; Dopey-2; C21orf5; DOP2_HUMAN; Dopey family member 2; DOPEY2; homolog of yeast DOP1; Protein dopey-2. |
| 研究领域 | 免疫学 神经生物学 信号转导 转运蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat (predicted: Human,Mouse,Pig,Sheep,Cow,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 258 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DOPEY2: 51-150/2298 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Dopey-2 is a 2,298 amino acid protein that is ubiquitously expressed with high levels found in the developing central nervous system where it is thought to play a role in protein trafficking between early endosomes and the late Golgi. Multiple isoforms of Dopey-2 exist due to alternative splicing events. The gene encoding Dopey-2 maps to human chromosome 21 and may be involved in the pathogenesis of Down syndrome. The smallest of the human chromosomes, chromosome 21 comprises about 1.5% of the human genome and contains nearly 300 genes and 47 million base pairs. Down syndrome, also known as trisomy 21, is the disease most commonly associated with chromosome 21. Alzheimer's disease, Jervell and Lange-Nielsen syndrome and amyotrophic lateral sclerosis are also associated with chromosome 21. Translocations are found to occur between chromosome 21 and 8, and chromosome 21 and 12, in certain leukemias. Function: May be involved in protein traffic between late Golgi and early endosomes. Tissue Specificity: Ubiquitously expressed. Overexpressed in lymphoblasts from Down syndrome patients. DISEASE: Abundantly expressed in developing central nervous system, with highest levels in cerebellum and lowest in telencephalon. Similarity: Belongs to the dopey family. SWISS: Q9Y3R5 Gene ID: 9980 Database links: Entrez Gene: 9980 Human Entrez Gene: 70028 Mouse Omim: 604803 Human SwissProt: Q9Y3R5 Human SwissProt: Q3UHQ6 Mouse Unigene: 204575 Human Unigene: 23230 Mouse |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DOPEY2) Polyclonal Antibody, Unconjugated (bs-14410R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
