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DOLPP1 Rabbit pAb (bs-14407R)  
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50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-14407R
英文名称 DOLPP1 Rabbit pAb
中文名称 长醇二磷酸酶1抗体
别    名 0610011H20Rik; AB030189; DOPP1_HUMAN; Dolichyldiphosphatase 1; Linked to Surfeit genes in Fugu rubripes 2; LSFR2; OTTHUMP00000022326; OTTHUMP00000196902.  
研究领域 细胞生物  转录调节因子  激酶和磷酸酶  
抗体来源 Rabbit
克隆类型 Polyclonal
克 隆 号
交叉反应 Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Chicken,Dog,Horse)
产品应用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 27 kDa
检测分子量
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human DOLPP1: 151-238/238 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 A similar gene has been characterized in mice and encodes dolichyl pyrophosphate (Dol-P-P) phosphatase. This protein dephosphorylates dolichyl pyrophosphate so that it may be re-utilized as a glycosyl carrier lipid by the oligosaccharyltransferase multisubunit complex in the ER. Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jun 2012]

Function:
DOLPP1 is a multi-pass membrane protein(By similarity). It belongs to the dolichyldiphosphatase family. It is required for efficient N-glycosylation and is necessary for maintaining optimal levels of dolichol-linked oligosaccharides. DOLPP1 hydrolyzes dolichyl pyrophosphate at a very high rate and dolichyl monophosphate at a much lower rate. It does not act on phosphatidate

Subcellular Location:
Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity).

Similarity:
Belongs to the dolichyldiphosphatase family.

SWISS:
Q86YN1

Gene ID:
57171

Database links:

Entrez Gene: 57171 Human

Entrez Gene: 57170 Mouse

Entrez Gene: 296624 Rat

SwissProt: Q86YN1 Human

SwissProt: Q9JMF7 Mouse



产品图片
Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-DOLPP1 (bs-14407R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 27 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DOLPP1) Polyclonal Antibody, Unconjugated (bs-14407R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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