| 产品编号 | bs-14278R |
| 英文名称 | DEPDC1B/BRCC3 Rabbit pAb |
| 中文名称 | 乳腺癌细胞3抗体 |
| 别 名 | BRCC3; Breast cancer cell 3; DEP domain containing 1B; DEP domain containing protein 1B; FLJ11252; DEP1B_HUMAN; HBV X transactivated gene 8 protein; HBV X-transactivated gene 8 protein; HBV XAg-transactivated protein 8; HBxAg transactivated protein 1; OTTHUMP00000122605; OTTHUMP00000221973; XTP1; XTP8. |
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Specific References (3) | bs-14278R has been referenced in 3 publications.
[IF=3.784] Z-D Liu. et al. Aberrantly high DEPDC1B expression leads to poor prognosis in patients with lower-grade gliomas. EUR REV MED PHARMACO. 2022 Nov;26(21):7813-7826 IHC ; Human.
[IF=1.664] Ma et al. High levels of glioma tumor suppressor candidate region gene 1 predicts a poor prognosis for prostate cancer. (2018) Oncol.Lett. 16:6749-6755 IHC ; Human.
[IF=1.39] Bai, Shoumin, et al. "High levels of DEPDC1B predict shorter biochemical recurrence‑free survival of patients with prostate cancer." Oncology Letters. WB, IHC-P ; Human.
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| 研究领域 | 肿瘤 细胞生物 信号转导 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit,Cow) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 62 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DEPDC1B/BRCC3: 451-529/529 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
GTPase activator activity. biological process: intracellular signaling cascade Subcellular Location: intracellular Similarity: Contains 1 DEP domain. Contains 1 Rho-GAP domain. SWISS: Q8WUY9 Gene ID: 55789 Database links: Entrez Gene: 55789 Human SwissProt: Q8WUY9 Human |
| 产品图片 |
Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human MCF-7 cell lysates
Primary: Anti-DEPDC1B/BRCC3 (bs-14278R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 68 kDa
Tissue/cell: human brain glioma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
