| 产品编号 | bs-10660R |
| 英文名称 | Rabbit Anti-SCP3 antibody |
| 中文名称 | 胆碱磷酸转移酶1抗体 |
| 别 名 | SCP3; choline phosphotransferase 1; chpt1; COR 1; COR1; MGC71888; RNASCP3; SCP 3; SCP-3; SPGF4; Sycp 3; Sycp3; SYCP3_HUMAN; Synaptonemal complex protein 3. |
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Specific References (1) | bs-10660R has been referenced in 1 publications.
[IF=6.208] Baohui Yao. et al. Reproductive Suppression Caused by Spermatogenic Arrest: Transcriptomic Evidence from a Non-Social Animal. INT J MOL SCI. 2023 Jan;24(5):4611 IHC ; Zokor.
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| 研究领域 | 细胞生物 染色质和核信号 细胞周期蛋白 细胞分化 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse (predicted: Human,Rat) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 27kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SYCP3: 151-236/236 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes an essential structural component of the synaptonemal complex. This complex is involved in synapsis, recombination and segregation of meiotic chromosomes. Mutations in this gene are associated with azoospermia in males and susceptibility to pregnancy loss in females. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, May 2010] Function: Component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Has an essential meiotic function in spermatogenesis. May be important for testis development. Required for efficient phosphorylation of HORMAD1 and HORMAD2. Subunit: Interacts with SYCP2. Subcellular Location: Nucleus. Chromosome. Note=In tripartite segments of synaptonemal complexes, irrespective of whether these are synapsed or unsynapsed. Tissue Specificity: Testis-specific. Post-translational modifications: Phosphorylated. DISEASE: Spermatogenic failure 4 (SPGF4) [MIM:270960]: An infertility disorder characterized by azoospermia, a condition of having no sperm present in the ejaculate. Testicular histology shows arrest of spermatogenesis at the pachytene stage of primary spermatocytes. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the XLR/SYCP3 family. SWISS: Q8IZU3 Gene ID: 50511 Database links: Entrez Gene: 50511 Human Entrez Gene: 20962 Mouse Omim: 604759 Human SwissProt: Q8IZU3 Human SwissProt: P70281 Mouse Unigene: 506504 Human Unigene: 297977 Mouse Unigene: 34889 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCP3) Polyclonal Antibody, Unconjugated (bs-10660R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
