| 产品编号 | bs-10659R |
| 英文名称 | Ephrin-B2 Rabbit pAb |
| 中文名称 | Ephrin-B2抗体 |
| 别 名 | EFN B2; EFNB 2; Efnb2; EFNB2_HUMAN; Eph related receptor tyrosine kinase ligand 5; EPH-related receptor tyrosine kinase ligand 5; ephrin B2; Ephrin-B2; EphrinB2; EPLG 5; EPLG5; Htk L; HTK ligand; HTK-L; HTKL; LERK 5; LERK-5; LERK5; Ligand of eph related kinase 5; MGC126226; MGC126227; MGC126228; OTTMUSP00000024973. |
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Specific References (3) | bs-10659R has been referenced in 3 publications.
[IF=3.743] Zhu F et al. EFNB2 facilitates cell proliferation, migration, and invasion in pancreatic ductal adenocarcinoma via the p53/p21 pathway and EMT. Biomed Pharmacother. 2020 Feb 6;125:109972. WB ; Human.
[IF=2.153] Sun Chen-Li. et al. Blockade of Erythropoietin-Producing Human Hepatocellular Carcinoma Receptor B1 in Spinal Dorsal Horn Alleviates Visceral Pain in Rats. Pain Res Manag. 2021;2021:7582494 WB ; Rat.
[IF=1.813] Huang Mi. et al. Icariin Alleviates Glucocorticoid-Induced Osteoporosis through EphB4/Ephrin-B2 Axis. Evid-Based Compl Alt. 2020;2020:2982480 IHC ; Mouse.
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| 研究领域 | 肿瘤 心血管 神经生物学 信号转导 生长因子和激素 激酶和磷酸酶 通道蛋白 细胞膜受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat (predicted: Human,Mouse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 34 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Ephrin-B2: 21-120/333 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors. [provided by RefSeq, Jul 2008] Function: Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons. Subunit: Interacts with PDZRN3. Binds to the receptor tyrosine kinases EPHA4, EPHB4 and EPHA3. Binds to Hendra virus and Nipah virus G protein. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Lung and kidney. Post-translational modifications: Inducible phosphorylation of tyrosine residues in the cytoplasmic domain. Similarity: Belongs to the ephrin family. Contains 1 ephrin RBD (ephrin receptor-binding) domain. SWISS: P52799 Gene ID: 1948 Database links: Entrez Gene: 1948 Human Entrez Gene: 13642 Mouse Omim: 600527 Human SwissProt: P52799 Human SwissProt: P52800 Mouse Unigene: 149239 Human Unigene: 209813 Mouse |
| 产品图片 |
Sample:
Lane 1: Rat Cerebrum tissue lysates
Lane 2: Rat Spleen tissue lysates
Primary: Anti-Ephrin-B2 (bs-10659R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 52 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
