| 产品编号 | bs-14001R |
| 英文名称 | ERPINA6/Cortisol Binding Globulin Rabbit pAb |
| 中文名称 | 皮质醇结合球蛋白抗体 |
| 别 名 | CBG; CBG_HUMAN; corticosteroid binding globulin; Corticosteroid-binding globulin; serine(or cysteine) proteinase inhibitor clade A(alpha 1 antiproteinase antitrypsin) member 6; serpin A6; serpin peptidase inhibitor clade A(alpha 1 antiproteinase antitrypsin) member 6; SERPINA6; transcortin. |
| 研究领域 | 肿瘤 转运蛋白 结合蛋白 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Sheep,Cow) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 43 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cortisol Binding Globulin: 121-220/405 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes an alpha-globulin protein with corticosteroid-binding properties. This is the major transport protein for glucorticoids and progestins in the blood of most vertebrates. The gene localizes to a chromosomal region containing several closely related serine protease inhibitors which may have evolved by duplication events. [provided by RefSeq, Jul 2008] Function: Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species. Subcellular Location: Secreted. Tissue Specificity: Plasma; synthesized in liver. Has also been identified in a number of glycocorticoid responsive cells. Post-translational modifications: N-glycosylated; binds 5 oligosaccharide chains. Glycosylation in position Asn-260 is needed for steroid binding. DISEASE: Defects in SERPINA6 are a cause of corticosteroid-binding globulin deficiency (CBG deficiency) [MIM:611489]. CBG deficiency is an extremely rare hereditary disorder characterized by reduced corticosteroid-binding capacity with normal or low plasma corticosteroid-binding globulin concentration, and normal or low basal cortisol levels associated with hypo/hypertension and muscle fatigue. Similarity: Belongs to the serpin family. SWISS: P08185 Gene ID: 866 Database links: Entrez Gene: 866 Human Omim: 122500 Human SwissProt: P08185 Human Unigene: 532635 Human |
| 产品图片 |
Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti- Cortisol Binding Globulin (bs-14001R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 73 kD
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ERPINA6 Polyclonal Antibody, Unconjugated (bs-14001R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
