| 产品编号 | bs-13939R |
| 英文名称 | CHST8 Rabbit pAb |
| 中文名称 | 碳水化合物磺基转移酶8抗体 |
| 别 名 | Carbohydrate(N acetylgalactosamine 4 0) sulfotransferase 8; Carbohydrate sulfotransferase 8; CHST 8; GalNAc 4 O sulfotransferase 1; GalNAc 4 ST1; CHST8_HUMAN; GalNAc4ST 1; GalNAc4ST; GALNAC4ST1; N acetylgalactosamine 4 O sulfotransferase 1; N acetylgalactosamine 4 O sulfotransferase. |
| 研究领域 | 细胞生物 生长因子和激素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 49 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CHST9: 141-240/424 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene belongs to the sulfotransferase 2 family. It is predominantly expressed in the pituitary gland, and is localized to the golgi membrane. This protein catalyzes the transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. It is responsible for sulfation of GalNAc on luteinizing hormone (LH), which is required for production of the sex hormones. Mice lacking this enzyme, exhibit increased levels of circulating LH, and precocious sexual maturation of both male and female mice. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2011] Function: CHST8, or Carbohydrate sulfotransferase 8, catalyzes the transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. It is required for biosynthesis of glycoprotein hormones lutropin and thyrotropin, by mediating sulfation of their carbohydrate structures. CHST8 is only active against terminal GalNAcbeta1 and GalNAcbeta and not toward chondroitin. Subcellular Location: Golgi apparatus membrane; Single-pass type II membrane protein. Similarity: Belongs to the sulfotransferase 2 family. SWISS: Q9H2A9 Gene ID: 64377 Database links: Entrez Gene: 64377 Human Entrez Gene: 68947 Mouse Omim: 610190 Human SwissProt: Q9H2A9 Human SwissProt: Q8BQ86 Mouse Unigene: 165724 Human Unigene: 29380 Mouse Unigene: 55646 Rat |
| 产品图片 |
Sample:Uterus (Mouse) Lysate at 40 ug
Primary: Anti-CHST8(bs-13939R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49kD
Observed band size: 59kD
Sample:Hela(human) Cell Lysate at 40 ug
Primary: Anti-CHST8(bs-13939R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49kD
Observed band size: 59kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
