| 产品编号 | bs-13919R |
| 英文名称 | CCK39 Rabbit pAb |
| 中文名称 | 胆囊收缩素39抗体 |
| 别 名 | CCK; CCK39; Cholecystokinin 39; Cholecystokinin; CCKN_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 神经生物学 信号转导 生长因子和激素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 11 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CCK39: 51-115/115 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Cholecystokinin is a brain/gut peptide. In the gut, it induces the release of pancreatic enzymes and the contraction of the gallbladder. In the brain, its physiologic role is unclear. The cholecystokinin pro-hormone is processed by endo- and exo-proteolytic cleavages. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Mar 2010] Function: Cholecystokinin (CCK) is one of the most important hormone regulators of the digestive process. The peptide is concentrated in the proximal small intestine (CCK cells) and is secreted into the blood in response to the ingestion of proteins and fats, where its actions include the stimulation of pancreatic secretion, the modulation of insulin output, the regulation of gastric emptying, and gallbladder contraction. In the central nervous system CCKergic fibres are widespread, being particularly abundant in the cerebral cortex. In some mesolimbic neurons projecting to the nucleus accumbens, CCK is colocalised with dopamine and it has been suggested that CCK might facilitate the function of dopamine in events such as stimulusreward associative behaviour. Secretion of CCK from the murine tumour cell line STC1 has been shown to be stimulated by pituitary adenylate cyclase activating protein (PACAP) and has raised the possibility that PACAP may function as a neuromodulator of CCK release from gut endocrine cells in vivo. Subunit: Binds to CCK-A receptors in the pancreas and CCK-B receptors in the brain. Subcellular Location: Secreted Post-translational modifications: The precursor is cleaved by proteases to produce a number of active cholecystokinins. Similarity: Belongs to the gastrin/cholecystokinin family. SWISS: P06307 Gene ID: 885 Database links: Entrez Gene: 885 Human Entrez Gene: 12424 Mouse Omim: 118440 Human SwissProt: P06307 Human SwissProt: P09240 Mouse SwissProt: Q53WS9 Mouse SwissProt: Q8R041 Mouse SwissProt: Q9DCL5 Mouse Unigene: 458426 Human Unigene: 9781 Rat |
| 产品图片 |
Tissue/cell: mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CCK 39 Polyclonal Antibody, Unconjugated(bs-13919R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CCK39) Polyclonal Antibody, Unconjugated (bs-13919R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
