| 产品编号 | bs-13842R |
| 英文名称 | Centaurin beta 2 |
| 中文名称 | CENTB2蛋白抗体 |
| 别 名 | ACAP 2; ACAP2; ACAP2_HUMAN; ANK repeat and PH domain-containing protein 2; Ankyrin repeat; Arf GAP with coiled coil ANK repeat and PH domains 2; ARF GAP with coiled coil; Arf-GAP with coiled-coil; CENT B 2; Centaurin beta2; Centaurin-beta-2; CENTB 2; CENTB2; Cnt b2; Cnt-b2; Cntb2; KIAA0041; Pleckstrin homology domains 2; Similar to yeast ZINC FINGER PROTEIN GCS1. |
| 研究领域 | 细胞生物 信号转导 细胞粘附分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 88kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Centaurin beta 2: 101-200/778 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The ADP-ribosylation factor (ARF) family of small GTP-binding proteins are involved in vesicular transport regulation and in controlling cytoskeletal organization and cell adhesion. These proteins mainly regulate membrane traffic. ACAP2 is a member of the centaurin GTPase-activating protein (GAP) family, which comprises a subset of ARF regulatory molecules that transduce PI 3-kinase activation into coordinated control of ARF-dependent pathways. ACAP1 and ACAP2 are both widely expressed in peripheral, tubular membranes and usually interact with each other in various tissues. GAP activity of both ACAP1 and ACAP2 is dependent upon phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2]. ACAP2 associates with ARF1 and ARF6. Overexpression of ACAP2 blocks the formation of ARF6-dependent protrusions. K1L is a protein required for growth of the Vaccinia Virus that interacts with the ankyrin repeats of ACAP2. Function: GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6). Tissue Specificity: Widely expressed. Highest level in lung. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Contains 3 ANK repeats. Contains 1 Arf-GAP domain. Contains 1 BAR domain. Contains 1 PH domain. SWISS: Q15057 Gene ID: 23527 Database links: Entrez Gene: 23527 Human Entrez Gene: 78618 Mouse GenBank: NM_030138 Mouse Omim: 607766 Human SwissProt: Q15057 Human SwissProt: Q3UHL4 Mouse SwissProt: Q6ZQK5 Mouse Unigene: 478746 Human Unigene: 593373 Human Unigene: 274646 Mouse Unigene: 208307 Rat |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
