| 产品编号 | bs-13734R |
| 英文名称 | Connexin 31.1 |
| 中文名称 | 间隙连接蛋白31.1/GJB5抗体 |
| 别 名 | Connexin 31.1; Connexin-31.1; Connexin31.1; Cx31.1; CXB5_HUMAN; Gap junction beta 5 protein; Gap junction beta-5 protein; Gap junction protein beta 5 31.1kDa; Gap junction protein beta 5; GJB 5; GJB5. |
| 研究领域 | 肿瘤 细胞生物 信号转导 细胞粘附分子 跨膜蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse (predicted: Human,Rat,Dog,Cow,Horse,Rabbit,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 31kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Connexin 31.1: 1-100/273 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The connexin family of proteins form hexameric complexes, called connexons, that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane ?helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino acid substitutions in the most highly conserved domains; the transmembrane and extracellular regions. Connexin 31.1, Cx31.1 or Gap junction beta-5 protein, is a 271 amino acid protein that is predominantly expressed in skin with lower expression in testis. Expression of connexin 31.1 is required for normal placental development in mice. Down-regulation of the connexin 31.1 gene correlates with head and neck squamous cell carcinomas (HNSCC) and therefore it may be a potential therapeutic target. Function: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Subcellular Location: Cell membrane. Cell junction > gap junction. Similarity: Belongs to the connexin family. Beta-type (group I) subfamily. SWISS: O95377 Gene ID: 2709 Database links: Entrez Gene: 2709 Human Omim: 604493 Human SwissProt: O95377 Human Unigene: 198249 Human |
| 产品图片 |
Sample:
Raw264.7(Mouse) Cell Lysate at 30 ug Primary: Anti- Connexin 31.1 (bs-13734R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 31 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
