| 产品编号 | bs-7351R |
| 英文名称 | Rabbit Anti-CD200R antibody |
| 中文名称 | CD200受体抗体 |
| 别 名 | CD 200R; CD200 cell surface glycoprotein receptor; CD200 receptor 1; Cd200r1; Cell surface glycoprotein CD200 receptor 1; Cell surface glycoprotein OX2 receptor 1; Cell surface glycoprotein OX2 receptor; cell surface glycoprotein receptor CD200; CRTR 2; CRTR2; HCRTR2; MO2R1_HUMAN; MOX2 receptor; MOX2R; OX2R. |
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Specific References (3) | bs-7351R has been referenced in 3 publications.
[IF=2.8] Clark, David A., et al. "Changes in expression of the CD200 tolerance‐signaling molecule and its receptor (CD200R) by villus trophoblasts during first trimester missed abortion and in chronic histiocytic intervillositis." American Journal of Reproductive Immunology (2017). IHC-P ; Human.
[IF=2.8] Clark, David A., et al. "The Receptor for the CD200 Tolerance‐Signaling Molecule Associated with Successful Pregnancy is Expressed by Early‐Stage Breast Cancer Cells in 80% of Patients and by Term Placental Trophoblasts." American Journal of Reproductive Immunology 74.5 (2015): 387-391. IHC-P ; Human.
[IF=2.8] Clark, David A., Jorge L. Arredondo, and Sukhbinder Dhesy-Thind. "The CD200 tolerance-signaling molecule and its receptor, CD200R1, are expressed in human placental villus trophoblast and in peri-implant decidua by 5 weeks’ gestation." Journal of Reproductive Immunology 112 (2015): 20-23. IHC-P ; Human.
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| 研究领域 | 细胞生物 免疫学 细胞膜受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=2μg/Test,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 34kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD200R: 151-250/325 <Extracellular> |
| 亚 型 | |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a receptor for the OX-2 membrane glycoprotein. Both the receptor and substrate are cell surface glycoproteins containing two immunoglobulin-like domains. This receptor is restricted to the surfaces of myeloid lineage cells and the receptor-substrate interaction may function as a myeloid downregulatory signal. Mouse studies of a related gene suggest that this interaction may control myeloid function in a tissue-specific manner. Alternative splicing of this gene results in multiple transcript variants. Function: Receptor for the CD200/OX2 cell surface glycoprotein. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200. Subcellular Location: Secreted and Cell membrane. Tissue Specificity: Expressed in granulocytes, monocytes, most T cells, neutrophils, basophils and a subset of NK, NKT and B cells (at protein level). Expressed in bone marrow, lymph nodes, spleen, lung, liver, spinal cord, kidney. Expressed in monocyte-derived dendritic and mast cells. Post-translational modifications: The mature form of isoform 2 and/or isoform 4 starts at sequence position 27 of the corresponding isoform. Similarity: Belongs to the CD200R family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q6Q8B3 Gene ID: 131450 Database links: Entrez Gene: 131450 Human Omim: 607546 Human SwissProt: Q8TD46 Human Unigene: 309158 Human |
| 产品图片 |
Sample:hela(Human) Cell Lysate at 30 ug
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-CD200R (bs-7351R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 58 kD
Paraformaldehyde-fixed, paraffin embedded (Human liver); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (CD200R) Polyclonal Antibody, Unconjugated (bs-7351R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Blank control:Hepg2 Cells(blue). Primary Antibody: Rabbit Anti-CD200R/AF647 Conjugated antibody (bs-7351R-AF647), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
