| 产品编号 | bs-13706R |
| 英文名称 | CLCA3 Rabbit pAb |
| 中文名称 | 钙激活氯离子通道蛋白3抗体 |
| 别 名 | Calcium activated chloride channel 3; Calcium-activated chloride channel regulator family member 3; Chloride channel accessory 3 pseudogene; chloride channel calcium activated 3; Chloride channel calcium activated family member 3; Clca1; Clca2; CLCA3P; Gob 5; Gob-5; Gob5; hCLCA3; mCLCA3; MGC143984; MGC143985. |
| 研究领域 | 肿瘤 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Mouse,Rat,Dog) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 98 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse CLCA3: 811-913/913 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The calcium-activated chloride channel (CLCA) protein family, which includes the human homologs CLCA1 and CLCA2, display distinct tissue distribution patterns. CLCA1 is expressed as a precursor protein that is processed into two cell surface associated subunits and a group of proteins. CLCA1 is upregulated by interleukin-9 and regulates the expression of mucins. CLCA1 may provide a therapeutic target to control mucus overproduction in airway disease patients with cystic fibrosis. CLCA2 expression is downregulated in breast cancer, therefore CLCA2 is thought to act as a tumor suppressor in normal cells. CLCA3 (known as Clca3 in mouse) is a structurally divergent member of the CLCA family that does not function as a channel protein. CLCA4 is a CLCA member that is expressed in human rectal mucosa, CLCA5 shows strong expression in eye and spleen, and CLCA6 is primarily expressed in intestine and stomach. Function: mCLCA3 (alias gob-5) is the third murine member of the family of calcium-activated chloride channels (CLCA-family). mCLCA3 plays a role in diseases with secretory dysfunctions, including asthma and cystic fibrosis. The mCLCA3 protein is located in mucin granule membranes of goblet cells of the intestinal, respiratory and reproductive tracts. It is also secreted into the mucin layer on mucosal membranes. The protein appears to be involved in the synthesis, condensation or secretion of mucins. Subcellular Location: Secreted Tissue Specificity: Exclusively expressed in the digestive and respiratory tracts and in the uterus (at protein level). Expressed in small intestine, colon, stomach, and uterus and slightly expressed in trachea tissue. Exclusively expressed in the mucin granule membranes of gastrointestinal, respiratory, and uterine goblet cells and other mucin-producing cells. In the colon, expressed in the surface mucous cells. In the stomach highly expressed in the surface epithelium in the pylorus. Strongly expressed in the airway epithelium of lung tissues associated with airway hyperresponsiveness (AHR). Post-translational modifications: he 110 kDa translation product is cleaved in the endoplasmic reticulum into a 75 kDa N-terminal and a 35 kDa C-terminal products that remain physically associated with each other. Glycosylated. Similarity: Belongs to the CLCR family. Contains 1 VWFA domain. SWISS: Q8R049 Gene ID: 23844 Database links: Entrez Gene: 23844 Mouse SwissProt: Q8R049 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
