| 产品编号 | bs-13657R |
| 英文名称 | NSP3 Rabbit pAb |
| 中文名称 | SH2结构域蛋白3A抗体 |
| 别 名 | CHAT; NSP3; PRO34088; SHEP1; SH2D3_HUMAN; SH2D3C; Cas/HEF1-associated signal transducer (Chat-H); Novel SH2-containing protein 3; SH2 domain-containing Eph receptor-binding protein 1 (SHEP1); SH2D3_MOUSE; Cas/HEF1-associated signal transducer; SH2 domain- |
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Specific References (1) | bs-13657R has been referenced in 1 publications.
[IF=2.401] Jie Wang . et al. BDNF-overexpressing human umbilical cord mesenchymal stem cell-derived motor neurons improve motor function and prolong survival in amyotrophic lateral sclerosis mice. Neurol Res. 2021;43(3):199-209 IF ; Human.
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| 研究领域 | 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human,Mouse,Rat,Cow,Dog) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 94 kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NSP3: 761-860/860 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Tumor necrosis factor (TNF) is a pleiotropic cytokine whose function is mediated through two distinct cell surface receptors (1,2). These receptors, designated TNF-R1 and TNF-R2, are expressed on most cell types (1,3). The majority of TNF functions are primarily mediated through TNF-R1 (1,4). FAN (for factor associated with neutral sphingomyelinase (N-SMase) activation) is an intermediate protein that interacts with TNF-R1 to initiate TNF signaling events. FAN binds to TNF-R1 at the cytoplasmic NSD (N-SMase activating domain), which results in the initiation of the N-SMase pathway (5). N-SMase has been shown to be involved in TNF-induced Raf-1 activation (6). FAN contains four carboxy-terminal WD-repeat domains which appear to be involved in protein-protein interaction. The FAN WD-repeats may mediate the interaction between FAN and TNF-R1 (5). Function: Eph receptor-binding protein which may be a positive regulator of TCR signaling. Binding to BCAR1 is required to induce membrane ruffling and promote EGF-dependent cell migration. Subcellular Location: Cytoplasm. Membrane. Associated with the membrane when EGF-stimulated. Found at ruffling membranes. Tissue Specificity: Ubiquitously expressed. Similarity: Contains 1 Ras-GEF domain. Contains 1 SH2 domain. SWISS: Q8N5H7 Gene ID: 10044 Database links: Entrez Gene: 10044 Human Entrez Gene: 27387 Mouse Omim: 604722 Human SwissProt: Q8N5H7 Human SwissProt: Q9QZS8 Mouse Unigene: 306412 Human Unigene: 9593 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
