| 产品编号 | bs-13594R |
| 英文名称 | DZIP1 |
| 中文名称 | 锌指蛋白DZIP1抗体 |
| 别 名 | DAZ interacting protein 1/2; DAZ interacting protein testis1; DZIP; DZIP2; DZIPt1; Zinc finger DAZ interacting protein 1; Zinc finger protein DZIP1; Zinc finger protein DZIPt1; DZIP1_HUMAN. |
| 研究领域 | 细胞生物 转录调节因子 锌指蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat (predicted: Human,Mouse,Dog,Pig,Cow,Horse,Rabbit) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 99kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DZIP1: 21-120/867 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
DZIP1 (DAZ interacting protein 1) is also known as DZIP or DZIP2 and is a 867 amino acid protein which is expressed as three isoforms, designated DZIPb, DZIPt1 and DZIPt2. DZIP1 is localized to testis, oocytes, ovary and fetal brain, as well as in embryonic stem cells and germ cells. In testis, DZIP1 is localized to the nucleus and also shows some cytoplasmic distribution in spermatogonia. DZIP1 belongs to the C2H2-type zinc-finger protein family, and, characteristc of the C2H2-type zinc-finger protein family, DZIP1 contains one C2H2-type zinc-finger region through which it is thought to interact with DAZ, an interaction that promotes spermiogenesis. DZIP1 expression is not found in those afflicted with Sertoli cell-only syndrome (characterized by the absence of germ cells in the testis), suggesting that the lack of DZIP1 may be involved in the pathogenesis of Sertoli cell-only syndrome-induced male sterility. Function: DZIP1 may participate in spermatogenesis via its interaction with DAZ. Isoform 1 and isoform 2 interact with DAZ proteins. DZIP1 may also participate in a RNA-binding complex that functions in both ES cells and germ cells. There are three named isoforms. Subcellular Location: Nucleus. Cytoplasm. Note: In testis, it is predominantly nuclear, with some cytoplasmic localization in spermatogonia. Co-localizes with DAZ and DAZL in primordial germ cells. Tissue Specificity: Predominantly expressed in testis. Also expressed in fetal brain, adult oocytes and ovary. Expressed in undifferentiated ES cells. In testis, it is probably specifically expressed in germ cells. Expressed in mature germ cells and secondary spermatocytes, while it is weakly or not expressed in primary spermatocytes. Similarity: Belongs to the DZIP C2H2-type zinc-finger protein family. Contains 1 C2H2-type zinc finger. SWISS: Q86YF9 Gene ID: 22873 Database links: Entrez Gene: 22873 Human Omim: 608671 Human SwissProt: Q86YF9 Human |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Rat ovary ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DZIP1) Polyclonal Antibody, Unconjugated (bs-13594R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DZIP1) Polyclonal Antibody, Unconjugated (bs-13594R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
