| 产品编号 | bs-15523R |
| 英文名称 | Rabbit Anti-phospho-IFNGR1 (Tyr457) antibody |
| 中文名称 | 磷酸化干扰素gamma受体1蛋白抗体 |
| 别 名 | IFNGR1 (phospho Y457);p-IFNGR1 (phospho Y457); Antiviral Protein Type II; Antiviral protein, type 2; AVP type II; AVP, type 2; CD 119; CD119; CDw119; CD119 antigen; CDw119; FLJ45734; IFN gamma R alpha; IFN gamma R; IFN gamma R1; IFN-gamma receptor 1; IFN-gamma-R1; IFNG R1; IFNGR 1; IFNGR; IFNGR1; Immune interferon receptor 1; Immune interferon receptor for; INGR1_HUMAN; Interferon gamma receptor 1; Interferon gamma receptor alpha chain; Interferon gamma receptor alpha chain precursor. |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 微生物学 干细胞 细菌及病毒 细胞表面分子 淋巴细胞 t-淋巴细胞 b-淋巴细胞 细胞因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 53kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human IFNGR1 around the phosphorylation site of Tyr457: EF(p-S)-NH2 |
| 亚 型 | |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene (IFNGR1) encodes the ligand-binding chain(alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection. [provided by RefSeq]. Function: Receptor for interferon gamma. Two receptors bind one interferon gamma dimer. Subunit: Monomer. Subcellular Location: Membrane; Single-pass type I membrane protein. Post-translational modifications: Phosphorylated at Ser/Thr residues. DISEASE: Mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]: This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the type II cytokine receptor family. Contains 2 fibronectin type-III domains. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. SWISS: P15260 Gene ID: 3459 Database links: Entrez Gene: 3459 Human Entrez Gene: 15979 Mouse Omim: 107470 Human SwissProt: P15260 Human SwissProt: P15261 Mouse Unigene: 520414 Human Unigene: 549 Mouse |
| 产品图片 |
Sample:Jurkat (Human)Cell Lysate at 40 ug
Primary: Anti-p-IFNGR1(bs-15523R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 53kD
Observed band size: 53kD
Sample: 293T Cell (Human) Lysate at 40 ug
Primary: Anti- phospho-IFNGR1 (Tyr457) (bs-15523R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Sample:
Panc-1(Human) Cell Lysate at 40 ug
A431 (Human) Cell Lysate at 40 ug
Primary: Anti-p-IFNGR1(bs-15523R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 53kD
Observed band size: 53kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-IFNGR1) Polyclonal Antibody, Unconjugated (bs-15523R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
